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The Earth Friendly%u00ae Company 5%u00aeQUALITY ICONSBIOLOGICAL TESTING & COMPLIANCE CERTIFICATION ICONSRefer to the icons that appear on each product page to determine which testing certifications apply to your product.AUTOCLAVING SPECIFICATIONSItems listed as autoclavable can be autoclaved at 122%u00b0 C for a maximum of 20 minutes at 15 PSI. Remember to loosen the caps from the tubes to prevent collapse when autoclaving. A steam cycle should be used. Always air dry these items. Avoid using a dry cycle or glassware cycle as deformation can occur to plastics that are heated for extended periods.SPIN RATE SPECIFICATIONRelative Centrifugal Force (RCF) is expressed as a multiple of the acceleration of gravity (g). The conversions of RCF and Revolutions Per Minute (RPM) can be calculated using the formulas: RCF = (RPM/1000)2 X r X 1.118; RPM = %u221a(RCF/(r X 1.118)) X 1000; r = centrifugal radius in mm = the distance from the centrifuge spindle to the bottom of the tube/bottom of rotor wells. This number can be measured and is also usually available %u2122 in the information provided with the rotor. Always run a trial to establish the RCF for the tube you intend to use.FREEZING SPECIFICATIONSMaximum nominal freezing temperatures are shown for specific products listed as Freezable. When freezing solutions, volume should not exceed 90% of the recommended capacity. Overfilled volume, crowding or inadequate spacing of tubes, and insulated racks can cause uneven cooling, cracking, or stress marks. PRODUCT OPERATION ICONSRefer to the icons that appear on each product page to determine which product is appropriate for your application.COMPLIANT WITH EU REGULATIONComplies with EU regulation 2017/746.CERTIFIED FREE OF DETECTABLE RNASE AND DNASE NUCLEASE ACTIVITYNuclease testing confirms the absence of RNase and DNase enzymes through the lack of degradation of DNA and RNA in an aqueous solution that has been incubated in Labcon products. DNase and RNase are examined by fluorescence-quenched oligonucleotide probes to confirm absence of these enzymes. CERTIFIED FREE OF ADENOSINE TRIPHOSPHATE (ATP)ATP testing of manufacturing processes and products can identify potential bacterial, fungal, and human sources of contamination. An extensive survey of Labcon products is used to confirm that the certified ATP-Free products are free of biological contamination. Results show less than 2x10-12 mg/%u03bcL.CERTIFIED FREE OF ENDOTOXIN (PYROGEN) CONTAMINATIONEndotoxin testing uses the Limulus Amebocyte Lysate coagulation assay to confirm the absence of neurotoxic, pyrogenic membrane lipids produced by bacterial species. This test confirms that Labcon products are free of bacterial contamination using an FDA suggested standard of 0.05 EU/mL as a maximum value.CERTIFIED FREE OF HEAVY METALS BELOW 1PPMProducts are tested for heavy metal content. All resins used for Labcon products are qualified to fall below one part per million for heavy metals.%u2122%u2122%u2122%u2122* Only specialty products include these biological tests and certifications** PCR is a patented process of Hoffman-LaRoche. Use of the PCR process requires a license.%u2122%u2122*CERTIFIED TO CONTAIN LESS THAN 1PPB OF 21 METALSUltra low metal products offered by Labcon are qualified to have less than one part per billion of the applicable elements using Inductively Coupled Plasma Mass Spectrometry (ICP-MS) testing.*CERTIFIED FREE OF DNAProducts are tested for DNA contamination using the Polymerase Chain Reaction (PCR) with primers known to amplify targets in the human genome. Parts must fall below 1 pg of detectable DNA to be certified as DNA-free.*CERTIFIED FREE OF PROTEASE ACTIVITYProtease testing confirms the absence of protein-degrading enzymes through exposure to 2ng of Proteinase K in an aqueous solution that has been incubated in Labcon products. Protein standards are examined by polyacrylamide gel electrophoresis to confirm molecular integrity. %u2122%u2122UltraLow TraceMetals%u2122Iconography ReferenceCertified Low TraceMetals%u2122 CERTIFIEDLow TraceMETALSCERTIFIED FREE OF PER- AND POLYFLUOROALK YL SUBSTANCES (PFAS)PFAS-free products offered by Labcon are qualified to have non-detectable levels of 62 distinct PFAS compounds, organic fluorine, and inorganic fluoride. PFOS, PFOA, PFNA, and PFHxS were among the diverse, fluorinated compounds in the target panel.